fa disassembly assays Search Results


fa disassembly assays  (Oxford Instruments)
99
Oxford Instruments fa disassembly assays
( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) <t>Disassembly</t> T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.
Fa Disassembly Assays, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fa+disassembly+assays/Imaris/pmc11957695-201-5-9
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fa disassembly assays - by Bioz Stars, 2026-09
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( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) Disassembly T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.

Journal: The Journal of Clinical Investigation

Article Title: Lysyl hydroxylase 2 glucosylates collagen VI to drive lung cancer progression

doi: 10.1172/JCI189197

Figure Lengend Snippet: ( A ) Areas of FAs (dots) in anti-p-paxillin–stained parental (P) and Col6-KR 344SQ cells (n ≥ 50 per group). ( B ) Representative TIRF micrograph of mCherry-paxillin-transfected 344SQ cells to detect FAs (arrows), Original magnification, ×60. ( C ) Disassembly T 1/2 of FAs (dots) in Col6-KR 344SQ cells determined based on 60 minute time-lapse sequences. ( D ) Areas of FAs (dots) in anti-p-paxillin–stained parental or LH2-WA 344SQ cells (n ≥ 50 per group). ( E ) Disassembly T 1/2 of FAs (dots) in parental and LH2-WA 344SQ cells determined based on 60 minute time-lapse sequences. ( F ) Quantification of activated ITGβ1 per cell (dot) by immunocytochemical analysis with antiactivated ITGβ1 antibody. ( G ) WB analysis of phospho- and total FAK in parental and Col6-KR 344SQ cells. Densitometric values normalized based on total FAK are under gel. ( H ) IP/WB analysis of Col6a3/ITGα2. WB analysis to detect Col6a3 was carried out on anti-ITGα2–immunoprecipitated proteins isolated from parental or Col6-KR 344SQ cells. Relative densitometric values are under gels. Total cell lysates (input). ( I ) Schematic illustration of findings. P values were determined using 1-way ANOVA ( A , D , and F ) or Student’s 2-tailed t test ( C and E ). **** P < 0.0001.

Article Snippet: Time-lapse videos were analyzed for FA disassembly assays in Imaris spots tracking module in Surpass mode.

Techniques: Staining, Transfection, Immunoprecipitation, Isolation